Recently added articles from Clinical Chemistry:
Low Testosterone and Risk of Premature Death in Older Men: Analytical and Preanalytical Issues in Measuring Circulating Testosterone
Jul 01, 2008; Platz, Elizabeth A ... Two recent papers in Circulation (1) and Journal of Clinical Endocrinology and Metabolism (2) reported that older men with lower serum testosterone concentrations had a greater risk of dying than did men with higher testosterone. This potentially clinically important finding was not observed in ...
Use of Biomarkers to Predict Cardiac Risk from Medications: Getting to the Heart of the Matter
Jul 01, 2008; Januzzi, James L Jr ... In May 1999, the US Food and Drug Administration approved rofecoxib, the first nonsteroidal antiinflammatory drug (NSAID)1 with specific cyclooxygenase II (COX-II) activity. Soon to follow were the approvals of other COX-II inhibitors, including celecoxib. The hypothetical advantage of COX-II ...
Cut Points for Lipids and Lipoproteins in Children and Adolescents: Should They Be Reassessed?
Jul 01, 2008; Kwiterovich, Peter O ... Antecedent plasma concentrations of lipids and Iipoproteins are associated with both early pathologic lesions of atherosclerosis and arterial changes in vivo, such as carotid intima media thickness (IMT)2 and endothelial dysfunction (1, 2). Healthy normolipidemic Finnish children, treated with a ...
Reporting Bias in Diagnostic and Prognostic Studies: Time for Action
Jul 01, 2008; Rifai, Nader; Altman, Douglas G; Bossuyt, Patrick M ... Truth is not only violated by falsehood; it may be equally outraged by silence. -Henri-Frédéric Amiel (1812-1881) Publication bias and selective reporting are recognized problems in the biomedical literature (1-3). Positive or favorable findings are more likely to be reported ...
Sequence-Specific Histone Methylation Is Detectable on Circulating Nucleosomes in Plasma
Jul 01, 2008; Deligezer, Ugur; Akisik, Ebru E; Erten, Nilgün; Dalay, Nejat ... BACKGROUND: Alterations in DNA methylation and histone modifications have been implicated in carcinogenesis. Although tumor-specific alterations in DNA methylation can be detected in the serum and plasma of cancer patients, no data are available on the presence of histone modifications in ...
New Horizons for Diagnostic Applications of Circulating Nucleosomes in Blood?
Jul 01, 2008; Holdenrieder, Stefan; Kolligs, Frank T; Stieber, Petra ... In 1974, Kornberg proposed a model of the organization of human chromatin as a nucleosomal chain formed by repeating histone-DNA sequences (1). Subsequent x-ray crystallographic analyses have confirmed his electron microscopy findings as advances in methodology and crystallographic resolution ...
Microarray Technology and Applications in the Arena of Genome-Wide Association
Jul 01, 2008; Grant, Struan F A; Hakonarson, Hakon ... BACKGROUND: There is a revolution occurring in single nucleotide polymorphism (SNP) genotyping technology, with high-throughput methods now allowing large numbers of SNPs (10^sup 5^-10^sup 6^) to be genotyped in large cohort studies. This has enabled large-scale genome-wide association (GWA) ...
Association between ESR2 Genetic Variants and Risk of Myocardial Infarction
Jul 01, 2008; Domingues-Montanari, Sophie; Subirana, Isaac; Tomás, Marta; Marrugat, Jaume; Senti, Mariano ... BACKGROUND: Environmental and genetic factors contribute to the development of complex diseases such as myocardial infarction (MI), the leading cause of death in men and women. Women develop MI approximately 10 years later than men, a difference that could be explained by the genes coding for ...
N-Terminal Pro-B-Type Natriuretic Peptide Concentrations Predict the Risk of Cardiovascular Adverse Events from Antiinflammatory Drugs: A Pilot Trial
Jul 01, 2008; Brune, Kay; Katus, Hugo A; Moecks, Joachim; Spanuth, Eberhard; Jaffe, Allan S; Giannitsis, Evangelos ... BACKGROUND: We investigated whether higher concentrations of N-terminal pro-B-type natriuretic peptide (NT-proBNP) predicts cardiovascular adverse events (CV-AEs) in patients with osteoarthritis treated with antiinflammatory drugs. METHODS: NT-proBNP was measured in baseline samples from ...
Retinol-Binding Protein 4 and Lipocalin-2 in Childhood and Adolescent Obesity: When Children Are Not Just "Small Adults"
Jul 01, 2008; Kanaka-Gantenbein, Christina; Margeli, Alexandra; Pervanidou, Panagiota; Sakka, Sophia; Mastorakos, George; Chrousos, George P; Papassotiriou, Ioannis ... BACKGROUND: Although there is much evidence regarding the physiologic and pathogenic roles of the newly described adipokines retinol-binding protein 4 (RBP4) and lipocalin-2 as potential promoters of insulin resistance in obese adults, relatively little information exists regarding their roles ...
Utility of Oligonucleotide Array-Based Comparative Genomic Hybridization for Detection of Target Gene Deletions
Jul 01, 2008; Wong, Lee-Jun C; Dimmock, David; Geraghty, Michael T; Quan, Richard; Lichter-Konecki, Uta; Wang, Jing; Brundage, Ellen K; Scaglia, Fernando; Chinault, A Craig ... BACKGROUND: Direct DNA sequencing is the primary clinical technique for identifying mutations in human disease, but sequencing often does not detect intragenic or whole-gene deletions. Oligonucleotide array-based comparative genomic hybridization (CGH) is currently in clinical use to detect ...
Measuring Carbohydrate-Deficient Transferrin by Direct Immunoassay: Factors Affecting Diagnostic Sensitivity for Excessive Alcohol Intake
Jul 01, 2008; Whitfield, John B; Dy, Veronica; Madden, Pamela A F; Heath, Andrew C; Martin, Nicholas G; Montgomery, Grant W ... BACKGROUND: Carbohydrate-deficient transferrin (CDT) is a marker of alcohol intake that is used for detecting or monitoring alcohol-use disorders. The introduction of a new direct immunoassay for CDT justifies reevaluation of test performance and reexamination of factors affecting test ...
Plasma Dehydroepiandrosterone and Risk of Myocardial Infarction in Women
Jul 01, 2008; Page, John H; Ma, Jing; Rexrode, Kathryn M; Rifai, Nader; Manson, JoAnn E; Hankinson, Susan E ... BACKGROUND: In this study we prospectively evaluated the relationships between plasma concentrations of dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) and subsequent myocardial infarction in women. METHODS: Using case-control sampling, we selected participants ...
Breast Cancer Diagnosis and Prognosis through Quantitative Measurements of Serum Glycan Profiles
Jul 01, 2008; Kyselova, Zuzana; Mechref, Yehia; Kang, Pilsoo; Goetz, John A; Dobrolecki, Lacey E; Sledge, George W; Schnaper, Lauren; Hickey, Robert J; Malkas, Linda H; Novotny, Milos V ... BACKGROUND: Glycosylated proteins play important roles in cell-to-cell interactions, immunosurveillance, and a variety of receptor-mediated and specific protein functions through a highly complex repertoire of glycan structures. Aberrant glycosylation has been implicated in cancer for many ...
Detection of APC Gene Deletions Using Quantitative Multiplex PCR of Short Fluorescent Fragments
Jul 01, 2008; Castellsagué, Ester; González, Sara; Nadal, Marga; Campos, Olga; Guinó, Elisabet; Urioste, Miguel; Blanco, Ignacio; Frebourg, Thierry; Capellá, Gabriel ... BACKGROUND: Approximately 20% of classic familial adenomatous polyposis (FAP) cases and 70% to 80% of attenuated FAP (AFAP) cases are negative for the APC/MUTYH point mutation. Quantitative multiplex PCR of short fluorescent fragments (QMPSF), a technique for detecting copy number alterations, ...
Estimating Glomerular Filtration Rate in Black South Africans by Use of the Modification of Diet in Renal Disease and Cockcroft-Gault Equations
Jul 01, 2008; van Deventer, Hendrick E; George, Jaya A; Paiker, Janice E; Becker, Piet J; Katz, Ivor J ... BACKGROUND: The 4-variable Modification of Diet in Renal Disease (4-v MDRD) and Cockcroft-Gault (CG) equations are commonly used for estimating glomerular filtration rate (GFR); however, neither of these equations has been validated in an indigenous African population. The aim of this study was ...
Relationship between Progression to AIDS and Thrombophilic Abnormalities in HIV Infection
Jul 01, 2008; Lijfering, Willem M; Sprenger, Herman G; Georg, Rita R; van der Meulen, Piet A; van der Meer, Jan ... BACKGROUND: HIV-infected patients are at increased risk of venous and arterial thrombosis. We hypothesized that acquired thrombophilic abnormalities that could predispose to thrombosis are most pronounced in patients in advanced stages of HIV infection. METHODS: We included 109 ...
Celiac Disease and IgA Deficiency: Complications of Serological Testing Approaches Encountered in the Clinic
Jul 01, 2008; McGowan, Kelly E; Lyon, Martha E; Butzner, J Decker ... BACKGROUND: IgA deficiency causes false-negative IgAbased celiac serology results in patients with celiac disease. Using a case-finding strategy, we examined the prevalence of IgA deficiency, physician evaluation, and management of IgA deficiency during serological testing for celiac ...
Highly Sensitive Cardiac Troponin T Values Remain Constant after Brief Exercise- or Pharmacologic-Induced Reversible Myocardial Ischemia
Jul 01, 2008; Kurz, Kerstin; Giannitsis, Evangelos; Zehelein, Joerg; Katus, Hugo A ... BACKGROUND: Using a new precommercial high-sensitivity cardiac troponin T (hsTnT) assay, we evaluated whether hsTnT increases after reversible myocardial ischemia. METHODS: In 195 patients undergoing nuclear stress testing (ST) using single-photon emission computed tomography (SPECT) for ...
Euthyroid Patient with Elevated Serum Free Thyroxine
Jul 01, 2008; van der Watt, George; Haarburger, David; Berman, Peter ... CASE A 32-year-old female patient presented complaining of increased flushing, perspiration and heat intolerance of 3 months' duration. Medical history included idiopathic thrombocytopenic purpura of 4 years' duration, which had been treated by splenectomy after failed immunosuppression ...