Recently added articles from Clinical Chemistry:
What Kind of Stroke Is It?
Dec 01, 2008; ... The search for protein biomarkers in peripheral blood to aid in the diagnosis of stroke and the prediction of prognosis after stroke and risk of future stroke is an active area of investigation. Although some markers, such as S100b and neuron-specific enolase, have been shown to have prognostic ...
Molecular Genotyping in Transfusion Medicine
Dec 01, 2008; ... "Blood-Matching Goes Genetic," a recent News Focus article in Science (1), illustrates the growing awareness of the impact of the genomics revolution on transfusion medicine and its potential to transform the way blood is selected for transfusion. Antibody-based technology has been the basis for ...
Newborn Screening for Wilson Disease: Does Liquid Chromatography-Tandem Mass Spectrometry Provide the Solution?
Dec 01, 2008; ... Detailed criteria for newborn screening programs have been formulated (1 ), but the basic principles include requirements that the screened disorder be medically serious, that effective therapy be available, and that the cost of detection plus treatment be justifiable (2). Wilson disease, a ...
Genetic Variations in UDP-Glucuronosyl Transferase 2B17: Implications for Testosterone Excretion Profiling and Doping Control Programs
Dec 01, 2008; ... The use of drugs and ergogenic substances to augment athletic performance, commonly referred to as doping, has evolved along with sporting events. Ancient Olympic athletes consumed mushrooms, plants, and herbs in an attempt to gain a competitive edge. The modern Olympic Games made their debut in ...
Emerging Biomarkers for the Diagnosis and Prognosis of Prostate Cancer
Dec 01, 2008; ... BACKGROUND: Early detection of prostate cancer (CaP), the most prevalent cancer and the second-leading cause of death in men, has proved difficult, and current detection methods are inadequate. Prostate-specific antigen (PSA) testing is a significant advance for early diagnosis of patients with ...
Tryptic Peptide Analysis of Ceruloplasmin in Dried Blood Spots Using Liquid Chromatography-Tandem Mass Spectrometry: Application to Newborn Screening
Dec 01, 2008; ... BACKGROUND: Newborn screening to identify infants with treatable congenital disorders is carried out worldwide. Recent tandem mass spectrometry (MS/ MS) applications have markedly expanded the ability to screen for >50 metabolic diseases with a single dried blood spot (DBS). The feature that ...
Lack of Observed Association between High Plasma Osteoprotegerin Concentrations and Ischemic Stroke Risk in a Healthy Population
Dec 01, 2008; ... BACKGROUND: Several studies suggest that osteoprotegerin (OPG) concentrations may be associated with the risk of ischemic stroke, but no large prospective studies have been conducted. We conducted a nested case-control study within a large cohort to elucidate a possible ...
Association of Lipoprotein-Associated Phospholipase A^sub 2^ Mass and Activity with Coronary and Aortic Atherosclerosis: Findings from the Dallas Heart Study
Dec 01, 2008; ... BACKGROUND: Our aim was to characterize the association of lipoprotein-associated phospholipase A^sub 2^ (Lp-PLA^sub 2^) with coronary and aortic atherosclerosis in a large population-based study. METHODS: Lp-PLA^sub 2^ mass and activity were measured in 2171 subjects 30-65 years old ...
Analytical Characteristics of Commercial Cardiac Troponin I and T Immunoassays in Serum from Rats, Dogs, and Monkeys with Induced Acute Myocardial Injury
Dec 01, 2008; ... BACKGROUND: Information is needed regarding analytical characteristics of cardiac troponin (cTn) assays used in preclinical studies. METHODS: We measured cTnI and cTnT in serum from normal animals and animals with induced myocardial injury [Sprague-Dawley (SD) and Wistar rats, beagle ...
Simultaneous Measurement of Plasma Concentrations of Proinsulin and C-Peptide and Their Ratio with a Trefoil-Type Time-Resolved Fluorescence Immunoassay
Dec 01, 2008; ... BACKGROUND: When the concentrations of 2 or more substances are measured separately, their molar ratios are subject to the additive imprecisions of the different assays. We hypothesized that the cumulative error for concentration ratios of peptides containing a common sequence might be minimized ...
Clinical Impact of New Prostate-Specific Antigen WHO Standardization on Biopsy Rates and Cancer Detection
Dec 01, 2008; ... BACKGROUND: Clinicians may be unaware that replacement of the historical total prostate-specific antigen (tPSA) standard with the WHO 96/670 international standard leads to difficulties in interpreting tPSA results. Our aim was to investigate the relationship between the Hybritech and WHO ...
Performance of a Single Assay for Both Type III and Type VI TMPRSS2:ERG Fusions in Noninvasive Prediction of Prostate Biopsy Outcome
Dec 01, 2008; ... BACKGROUND: TMPRSS2:ERG fusions are promising prostate cancer biomarkers. Because they can occur in multiple forms in a single cancer specimen, we developed a quantitative PCR test that detects both type III and type VI TMPRSS2:ERG fusions. The assay is quantified from a standard curve ...
Meconium Nicotine and Metabolites by Liquid Chromatography-Tandem Mass Spectrometry: Differentiation of Passive and Nonexposure and Correlation with Neonatal Outcome Measures
Dec 01, 2008; ... BACKGROUND: Meconium analysis is a diagnostically sensitive and objective alternative to maternal self-report for detecting prenatal tobacco exposure. Nicotine and metabolite disposition in meconium is poorly characterized, and correlation of analytes' concentrations with neonatal outcomes is ...
Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection
Dec 01, 2008; ... BACKGROUND: Measurement of methylmalonic acid (MMA) in serum or plasma is useful for diagnosing cobalamin deficiency. We developed a method for quantifying MMA in plasma based on hydrophilic interaction liquid chromatography (HILIC) and single-stage negative electrospray ionization (ESI) mass ...
Newborn Screening for Sickle Cell Disease Using Tandem Mass Spectrometry
Dec 01, 2008; ... BACKGROUND: Neonatal screening programs for sickle cell disease are now widespread in North American and European countries. Most programs apply isoelectric focusing or HPLC to detect hemoglobin variants. Because tandem mass spectrometry (MS/MS) is being used for screening of inherited metabolic ...
Prediction of Recurrent Venous Thromboembolism by Endogenous Thrombin Potential and D-Dimer
Dec 01, 2008; ... BACKGROUND: Increased thrombin generation is associated with an increased risk of recurrent venous thromboembolism. We investigated the relation between endogenous thrombin potential (ETP) and risk of recurrent venous thromboembolism and evaluated whether prediction of recurrence can be improved ...
Assessing the Impact of the Frequency of Quality Control Testing on the Quality of Reported Patient Results
Dec 01, 2008; ... BACKGROUND: The traditional measure used to evaluate QC performance is the probability of rejecting an analytical run that contains a critical out-of-control error condition. The probability of rejecting an analytical run, however, is not affected by changes in QC-testing frequency. A different ...
High-Resolution Melting Curve Analysis of Genomic and Whole-Genome Amplified DNA
Dec 01, 2008; ... BACKGROUND: High-resolution melting curve analysis is an accurate method for mutation detection in genomic DNA. Few studies have compared the performance of high-resolution DNA melting curve analysis (HRM) in genomic and whole-genome amplified (WGA) DNA. METHODS: In 39 paired genomic and ...
Automated Measurement of 25-OH Vitamin D^sub 3^ on the Roche Modular E170 Analyzer
Dec 01, 2008; ... BACKGROUND: The first commercial direct automated immunoassay specific for 25-OH vitamin D^sub 3^ (25-OH-D^sub 3^) was recently introduced for use on Roche Diagnostics immunoassay analyzers. We assessed the analytical performance of the Elecsys 25-OH-D^sub 3^ assay on a Roche Modular E 170 ...
Seminal Oligouridinosis: Low Uridine Secretion as a Biomarker for Infertility in Spinal Neurotrauma
Dec 01, 2008; ... BACKGROUND: Compromised sexual health is a major rehabilitative barrier for men with lower-spinal cord injury (SCI). Although studies have revealed decreased sperm motility, the quantitative biochemical changes that underlie the infertility mechanism remain poorly understood. METHODS: We ...