Recently added articles from Photochemistry and Photobiology:
Amino Acid Residues in GRK1/GRK7 Responsible for Interaction with S-Modulin/Recoverin[dagger]
Jul 01, 2008; Torisawa, Aya; Arinobu, Daisuke; Tachibanaki, Shuji; Kawamura, Satoru ... ABSTRACT GRK1 is a visual pigment kinase in rods and is essential for inactivation of light-activated rhodopsin. The GRK1 activity is inhibited by binding of the Ca^sup 2+^-bound form of S-modulin/recoverin. We previously identified the S-modulin/recoverin site to interact with GRK1. In ...
Introduction to the Symposium-in-Print on Photoreceptors and Signal Transduction in Honor of Professor Fumio Tokunaga
Jul 01, 2008; Hisatomi, Osamu; Iwasa, Tatsuo; Kataoka, Mikio; Ebrey, Thomas ... Fumio Tokunaga has been a major leader in photobiology research for almost 40 years; his interests have been particularly broad, ranging from his early work on the effect of UV on DNA to those studies for which he is best known: on visual pigments and their associated enzymes, bacteriorhodopsin, ...
Salamander Blue-sensitive Cones Lost During Metamorphosis[dagger]
Jul 01, 2008; Chen, Ying; Znoiko, Sergey; DeGrip, Willem J; Crouch, Rosalie K; Ma, Jian-xing ... ABSTRACT The tiger salamander lives in shallow water with bright light in the aquatic phase, and in dim tunnels or caves in the terrestrial phase. In the aquatic phase, there are five types of photoreceptors-two types of rods and three types of cones. Our previous studies showed that the ...
Quantum Mechanical/Molecular Mechanical Studies on Spectral Tuning Mechanisms of Visual Pigments and Other Photoactive Proteins[dagger]
Jul 01, 2008; Altun, Ahmet; Yokoyama, Shozo; Morokuma, Keiji ... ABSTRACT The protein environments surrounding the retinal tune electronic absorption maximum from 350 to 630 nm. Hybrid quantum mechanical/molecular mechanical (QM/MM) methods can be used in calculating excitation energies of retinal in its native protein environments and in studying the ...
Role of the N-terminal Region in the Function of the Photosynthetic Bacterium Transcription Regulator PpsR[dagger]
Jul 01, 2008; Yamazaki, Yoichi; Fukusumi, Hayato; Kamikubo, Hironari; Kataoka, Mikio ... ABSTRACT PpsR is a transcription represser for the gene cluster encoding photosystem genes in Rhodobacter sphaeroides. Repression activity is accomplished by DNA binding on the promoter regions of the photosystem gene clusters, and depends on both the redox potential and the presence of ...
Synthesis and Spectroscopic Characterization of Photo-affinity Peptide Ligands to Study Rhodopsin-G Protein Interaction[dagger]
Jul 01, 2008; Chen, Yihui; Herrmann, Rolf; Fishkin, Nathan; Henklein, Peter; Nakanishi, Koji; Ernst, Oliver P ... ABSTRACT G protein-coupled receptors (GPCRs) are involved in the control of virtually all aspects of our behavior and physiology. Activated receptors catalyze nucleotide exchange in heterotrimeric G proteins (composed of α-GDP, β and γ subunits) on the inner surface of the ...
Signal Transfer in Haloarchaeal Sensory Rhodopsin-Transducer Complexes[dagger]
Jul 01, 2008; Sasaki, Jun; Spudich, John L ... ABSTRACT Membrane-inserted complexes consisting of two photochemically reactive sensory rhodopsin (SR) subunits flanking a homodimer of a transducing protein subunit (Htr) are used by halophilic archaea for sensing light gradients to modulate their swimming behavior (phototaxis). The ...
The Photoreaction of the Photoactive Yellow Protein Domain in the Light Sensor Histidine Kinase Ppr is Influenced by the C-terminal Domains[dagger]
Jul 01, 2008; Kamikubo, Hironari; Koyama, Tomonori; Hayashi, Michihiro; Shirai, Kumiko; Yamazaki, Yoichi; Imamoto, Yasushi; Kataoka, Mikio ... ABSTRACT To study the role of the C-terminal domains in the photocycle of a light sensor histidine kinase (Ppr) having a photoactive yellow protein (PYP) domain as the photosensor domain, we analyzed the photocycles of the PYP domain of Ppr (Ppr-PYP) and full-length Ppr. The gene ...
Alpha-retinals as Rhodopsin Chromophores-Preference for the 9-Z Configuration and Partial Agonist Activity[dagger]
Jul 01, 2008; Wang, Yajie; Bovee-Geurts, Petra H M; Lugtenburg, Johan; DeGrip, Willem J ... ABSTRACT The visual pigment rhodopsin, the photosensory element of the rod photoreceptor cell in the vertebrate retina, shows in combination with an endogenous ligand, 11-Z, retinal, an astonishing photochemical performance. It exhibits an unprecedented quantum yield (0.67) in a highly ...
Protein Diffusion Probed by the Transient Grating Method with a New Type of Photochromic Molecule[dagger]
Jul 01, 2008; Eitoku, Takeshi; Terazima, Masahide ... ABSTRACT A new type of photochromic molecule that can be used for diffusion coefficient (D) measurements of various proteins in solution is described. The absorption spectrum of this molecule is changed upon photoexcitation by the trans-cis isomerization reaction. Target proteins were ...
Dynamics Change of Phoborhodopsin and Transducer by Activation: Study Using D75N Mutant of the Receptor by Site-directed Solid-state ^sup 13^C NMR[dagger]
Jul 01, 2008; Kawamura, Izuru; Yoshida, Hideaki; Ikeda, Yoichi; Yamaguchi, Satoru; Tuzi, Satoru; Saitô, Hazime; Kamo, Naoki; Naito, Akira ... ABSTRACT Pharaonis phoborhodopsin (ppR or sensory rhodopsin II) is a negative phototaxis receptor of Natronomonas pharaonis, and forms a complex, which transmits the photosignal into cytoplasm, with its cognate transducer (pHtrII). We examined a possible local dynamics change of ppR and ...
Activity Switches of Rhodopsin[dagger]
Jul 01, 2008; Ritter, Eglof; Elgeti, Matthias; Bartl, Franz J ... ABSTRACT Rhodopsin, the usual pigment of the rod photoreceptor cell contains as its light-sensitive cofactor 11-cis retinal, which is bound by a protonated Schiff base between its aldehyde group and the Lys296 side chain of the apoprotein. Light activation is achieved by 11-cis all-trans ...
Protein-Protein Interaction of a Pharaonis Halorhodopsin Mutant Forming a Complex with Pharaonis Halobacterial Transducer Protein II Detected by Fourier-Transform Infrared Spectroscopy[dagger]
Jul 01, 2008; Furutani, Yuji; Ito, Motohiro; Sudo, Yuki; Kamo, Naoki; Kandori, Hideki ... ABSTRACT Pharaonis halorhodopsin (pHR) functions as a light-driven inward chloride ion pump in Natoronomonas pharaonis, while pharaonis phoborhodopsin (ppR; also called pharaonis sensory rhodopsin II, pSRII), is a light sensor for negative phototaxis. ppR forms a 2:2 complex with its ...
First Cytoplasmic Loop of Glucagon-like Peptide-1 Receptor Can Function at the Third Cytoplasmic Loop Position of Rhodopsin[dagger]
Jul 01, 2008; Yamashita, Takahiro; Tose, Koji; Shichida, Yoshinori ... ABSTRACT G protein-coupled receptors (GPCRs) are classified into several families based on their amino acid sequences. In family 1, GPCRs such as rhodopsin and adrenergic receptor, the structure-function relationship has been extensively investigated to demonstrate that exposure of the ...
The Lifetimes of Pharaonis Phoborhodopsin Signaling States Depend on the Rates of Proton Transfers-Effects of Hydrostatic Pressure and Stopped Flow Experiments[dagger]
Jul 01, 2008; Kikukawa, Takashi; Saha, Chabita K; Balashov, Sergei P; Imasheva, Eleonora S; Zaslavsky, Dmitry; Gennis, Robert B; Abe, Takayuki; Kamo, Naoki ... ABSTRACT Pharaonis phoborhodopsin (ppR), a negative phototaxis receptor of Natronomonas pharaonis, undergoes photocycle similar to the light-driven proton pump bacteriorhodopsin (BR), but the turnover rate is much slower due to much longer lifetimes of the M and O intermediates. The M ...
Ca^sup 2+^-dependent Regulation of Phototransduction[dagger]
Jul 01, 2008; Stephen, Ricardo; Filipek, Slawomir; Palczewski, Krzysztof; Sousa, Marcelo Carlos ... ABSTRACT Photon absorption by rhodopsin triggers the phototransduction signaling pathway that culminates in degradation of cGMP, closure of cGMP-gated ion channels and hyperpolarization of the photoreceptor membrane. This process is accompanied by a decrease in free Ca^sup 2+^ ...
Transient Vibronic Structure in Ultrafast Fluorescence Spectra of Photoactive Yellow Protein[dagger]
Jul 01, 2008; Nakamura, Ryosuke; Hamada, Norio; Ichida, Hideki; Tokunaga, Fumio; Kanematsu, Yasuo ... ABSTRACT The ultrafast photo-induced dynamics of wild-type photoactive yellow protein and its site-directed mutant of E46Q in aqueous solution was studied at room temperature by femtosecond fluorescence spectroscopy using the optical Kerr-gate method. The vibronic structure appears, ...
Low-temperature Spectroscopy of Met100Ala Mutant of Photoactive Yellow Protein[dagger][double dagger]
Jul 01, 2008; Imamoto, Yasushi; Harigai, Miki; Morimoto, Takashi; Kataoka, Mikio ... ABSTRACT The trans-to-cis photoisomerization of the p-coumaroyl chromophore of photoactive yellow protein (PYP) triggers the photocycle. Met100, which is located in the vicinity of the chromophore, is a key residue for the cis-to-trans back-isomerization of the chromophore, which is a ...
Presence of Rhodopsin and Porphyropsin in the Eyes of 164 Fishes, Representing Marine, Diadromous, Coastal and Freshwater Species-A Qualitative and Comparative Study[dagger]
Jul 01, 2008; Toyama, Mina; Hironaka, Mantaro; Yamahama, Yumi; Horiguchi, Hiroko; Tsukada, Osamu; Uto, Norihiko; Ueno, Yuka; Tokunaga, Fumio; Seno, Keiji; Hariyama, Takahiko ... ABSTRACT There are two types of visual pigments in fish eyes; most marine fishes have rhodopsin, while most freshwater fishes have porphyropsin. The biochemical basis for this dichotomy is the nature of the chromophores, retinal (A1) and 3-dehydroretinal (A2), each of which is bound by ...
Rhodopsin Regeneration is Accelerated via Noncovalent 11-cis Retinal-Opsin Complex-A Role of Retinal Binding Pocket of Opsin[dagger]
Jul 01, 2008; Matsumoto, Hiroyuki; Yoshizawa, Tôru ... ABSTRACT The regeneration of bovine rhodopsin from its apoprotein opsin and the prosthetic group 11-cis retinal involves the formation of a retinylidene Schiff base with the ε-amino group of the active lysine residue of opsin. The pH dependence of a Schiff base formation in solution ...