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High quality copy number and genotype data from FFPE samples using Molecular Inversion Probe (MIP) microarrays.(Technical advance)(Report)
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BMC Medical Genomics; Feb 19, 2009; ; 700+ Words
...Moorhead [1]; Zhigang C Wang [3]; Andrea L Richardson [3]; Robert...vast collection of stored FFPE samples already exists...suggests that ~500 million FFPE samples may have been collected...CN and genotype data from FFPE samples [4, 5, 6, 7...
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Improved RNA quality and TaqMan.sup.[R] .sup.Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials.(Research article)(ribonucleic acid)
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BMC Biotechnology; Feb 6, 2008; ; 700+ Words
...extracts and even from FFPE containing partially...invariably higher C T values in the latter...produced decreased C T s (e.g. with...RNA extracted from FFPE (Figure 3). Figure...at 70[degrees]C for 20 minutes...contained 1 [mu]l of extracted RNA...
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PCR-ready DNA from FFPE tissue in 30 minutes.(Biochemicals & Kits: Application Note)
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Bioscience Technology; Oct 1, 2006; ; 700+ Words
...paraffin-embedding (FFPE). In recent years...extraction of DNA from FFPE tissues. The DNA can...PCR (qPCR). Methods FFPE tissue from a 2-month...blade into 100 [micro]l of QuickExtract Solution and heated at 65 C. At 0.5, 1, and...
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Targeted Gene-Expression Analysis by Genome-Controlled Reverse Transcription-PCR
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Clinical Chemistry; Nov 1, 2006; ; 700+ Words
...well as in archival FFPE tissues. 2006 American...paraffin-embedded (FFPE) tissue samples (7...the fragmented mRNA in FFPE samples (4), RT...cells were grown at 37 C in a humidified atmosphere...bovine serum, 2 mmol/L L-glutamine, 100...
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Expression profiling with RNA from formalin-fixed, paraffin-embedded material.(Technical advance)(messenger ribonucleic acid)
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BMC Medical Genomics; Apr 19, 2008; ; 700+ Words
...for RNA isolation from FFPE material and an optimized...embedding in paraffin. FFPE material was obtained...at -80[degrees]C. Fresh frozen material...Bern. Both, FF and FFPE samples were checked...homogenized in 420 [mu]l lysis buffer (4 M guanidinium...
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RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies.(Methodology article)(ribonucleic acid )
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BMC Molecular Biology; Dec 21, 2007; ; 700+ Words
...paraffin-embedded (FFPE) samples are universally...RNA extraction from FFPE samples in 1988 [3...60 to 70[degrees]C) also removes part...that fragmentation of FFPE tissue continues even...quantity (in pg/[mu]l) and quality (by RNA...
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CDKN1C /p57.sup.kip2 .sup.is a candidate tumor suppressor gene in human breast cancer.(Research article)
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BMC Cancer; Mar 6, 2008; ; 700+ Words
...contributor) [1]; Benjamin L Schlechter (equal contributor...Morenas [1]; Carol L Rosenberg (corresponding...and paraffin embedded (FFPE). In 10 cases, tissues...stored at -80[degrees]C. One or more experienced...previously [38, 39, 40]. FFPE or OCT embedded blocks...
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Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material.(Methodology)
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Diagnostic Pathology; Sep 27, 2007; ; 700+ Words
...contributor) [1]; Alfred C Feller [1]; Hartmut...screening of blood and FFPE samples. In this publication...paraffin-embedded tissue (FFPE) from five patients...final volume of 25 [mu]l. Primer combination...Temperature 57[degrees]C) MastoMutF1: 5...
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Transmission of equine influenza virus to English foxhounds.(DISPATCHES)
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Emerging Infectious Diseases; Mar 1, 2008; ; 700+ Words
...obtained during the first visit to pack l (Table). Of these, 8 were from hounds...formalin-fixed paraffin-embedded (FFPE) tissues from the 2 hounds that were...negative. Deparaffinization of the FFPE lung tissue from the 2 hounds was performed...
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Increased cell motility and invasion upon knockdown of lipolysis stimulated lipoprotein receptor (LSR) in SW780 bladder cancer cells.(Research article)
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BMC Medical Genomics; Jul 22, 2008; ; 700+ Words
...antibodies were raised against the peptide [C]VTSLHEDDWRSRPSR, aa 386-400, BC000015...m formalin fixed paraffin embedded (FFPE) sections of histological specimens as...scored independently by two observers (L.D and M.H). Staining categories...
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New Technology Showcase
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Biopharm International; Dec 1, 2008; ; 700+ Words
...viral, or formalin-fixed, paraffin-embedded (FFPE) nucleic acid samples. SPRITE incorporates Beckman...connections, filters, and bag sizes (from 50 mL to 50 L). Many designs incorporate C-Flex TPE tubing, allowing the use of BioWelder and...
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