Article: Analysis of nuclear-cytoplasmic translocation using high throughput microscopy: real-time investigation of cellular and sub-cellular processes.(Tools & Techniques)

Abstract

It is increasingly recognized that gaining a detailed understanding of complex biological systems such as cellular pathways is fundamental to advances in basic research and drag discovery. Most importantly, there is a need for the direct visualization of complex cellular and sub-cellular processes that serve to improve early drug discovery and basic research. The EIDAQ[TM] 100 HTM cellular imaging and analysis system facilitates accurate investigation of cellular and sub-cellular processes in real time. Here we describe how image analysis using the system is applied to quantify intracellular translocation of the transcription factor NF-[kappa]B. ...

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