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Article: Methods for the detection of trace metabolites from biotransformation of polycyclic aromatic hydrocarbons by yeast.
- Article from:
- Journal of the Tennessee Academy of Science
- Article date:
- July 1, 2003
- Author:
CopyrightCOPYRIGHT 2003 Tennessee Academy of Science. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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ABSTRACT--The analysis of pollutants and their metabolites resulting from biotransformation is important to studies of bioremediation or toxicology of environmental toxicants. Gas chromatography-mass spectrometry (GC-MS) with solid-phase micro-extraction (SPME) has been used to determine the 9-hydroxyphenanthrene levels resulting from phenanthrene biotransformation by yeast. Candida tropicalis CP1-1, derived from a parent strain of Candida tropicalis (ATCC strain 96745), was grown in minimal media containing phenanthrene. Direct extraction of liquid media by SPME served to selectively preconcentrate the pollutants prior to GC-MS analysis. Various derivatizing reagents, ...