Article: Diversa obtains United States patent.(Brief Article)

Diversa Corporation (San Diego, CA) has patented methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution. A particular advantage of exonuclease-mediated reassembly methods is the ability to reassemble nucleic acid strands that would otherwise be problematic to chimerize. Exonuclease-mediated reassembly methods can be used in combination with other mutagenesis methods provided herein. These methods include non-stochastic polynucleotide site-saturation mutagenesis and non-stochastic polynucleotide reassembly. This invention provides methods of obtaining novel enzymes that have optimized physical &/or ...

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