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Article: Human DNA polymerase-eta is a reverse transcriptase.
- Article from:
- Hematology Week
- Article date:
- June 14, 2004
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2004 JUN 14 - (NewsRx.com & NewsRx.net) -- Human DNA polymerase-eta is a reverse transcriptase.
"We have proposed previously that error-prone reverse transcription using pre-mRNA of rearranged immunoglobulin variable (IgV) regions as templates is involved in the antibody diversifying mechanism of somatic hypermutation (SHM). As patients deficient in DNA polymerase-eta exhibit an abnormal spectrum of SHM, we postulated that this recently discovered Y-family polymerase is a reverse transcriptase (RT)," investigators in Australia wrote.
"This possibility was tested using a product-enhanced RT (PERT) assay that uses a real time PCR step with a fluorescent ...