|
|
Article: Isocratic separation of proteins using a thermal gradient.(Tools & Techniques)
- Article from:
- Bioscience Technology
- Article date:
- December 1, 2004
CopyrightCOPYRIGHT 2004 Advantage Business Media. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
|
Introduction
HPLC analysis at elevated temperatures reduces analysis time and improves resolution. Reduced viscosity at elevated temperatures results in significantly lower backpressure, allowing for high flow rates and short analysis times. It also permits the use of smaller particle size packings to increase efficiency while operating at lower backpressure. Flatter van Deemter curves mean that operating at higher flow rates does not sacrifice efficiency. Solvent properties also change as the temperature is increased. Hydrogen bonding interactions in water are reduced at higher temperatures, making water less polar so that it behaves more like an organic solvent ...