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Semilinear high-dimensional model for normalization of microarray data: a theoretical analysis and partial consistency; Comment.
- Article from:
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Journal of the American Statistical Association
- Article date:
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September 1, 2005
- Author:
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Copyright informationCOPYRIGHT 2005 American Statistical Association. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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1. INTRODUCTION
I would like to begin by congratulating Fan, Peng, and Huang on their innovative approach to normalizing spotted cDNA microarray data. Although numerous articles have been written on this topic, the use of duplicate spots in the normalization process has rarely been discussed. Given the apparent within-slide spatial effects (Smyth, Yang, and Speed 2003; Balazsi, Kay, Barabasi, and Oltvai 2003), these spots have the potential to be very helpful not only in the normalization process itself, but also in assessing the effectiveness of a normalization procedure. Unfortunately, the focus of the article limits the discussion of this approach and leaves several ...
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