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Article: Comparison of ultrastructural cytotoxic effects of carbon and carbon/iron particulates on human monocyte-derived macrophages.(Reasearch)
- Article from:
- Environmental Health Perspectives
- Article date:
- February 1, 2005
- Author:
CopyrightCOPYRIGHT 2005 National Institute of Environmental Health Sciences. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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In this study, we tested the hypothesis that the presence of iron in carbon particulates enhances ultrastructural perturbation in human monocyte-derived macrophages (MDMs) after phagocytosis. We used 1-[micro]m synthetic carbon-based particulates, designed to simulate environmental particulates of mass median aerodynamic diameter [less than or equal to] 2.5 [micro]m (P[M.sub.2.5]). Cultures of human MDMs or T-lymphocytes (as a nonphagocytic control) were exposed to carbon or carbon/iron particulates for various time periods and examined by transmission electron microscopy for ultrastructural changes. T-cells failed to internalize either of the particulates and showed no ...
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