Article: Quantitative HPLC analysis of lipids: the charged aerosol detector detected common lipids such as triacylglycerols, cholesterol esters, and free sterols to a minimum limit of detection of about 1 nanogram per injection. The mass to peak area ratio was nearly linear from about 1 nanogram to 10 micrograms per injection.(APPLICATION Note)

A recent study by Dr. Robert A. Moreau, of the Eastern Regional Research Center of the Agricultural Research Service, USDA, evaluated a charged aerosol detector (CAD), a new type of liquid chromatography detector, (1) with several normal phase and reverse phase HPLC systems commonly used for the quantitative analysis of lipid classes and lipid molecular species. The CAD detected common lipids such as triacylglycerols, cholesterol esters, and free sterols to a minimum limit of detection of about 1 nanogram per injection. The mass to peak area ratio was nearly linear from about 1 nanogram to 10 micrograms per injection.

Quantitative analysis of lipids becoming more ...

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