Article: The Next Era of Gene Expression Tools.(drug discovery and gene expression)

By Bruce Seligmann, PhD

Obstacles to measuring gene expression have hindered the impact of genomics on drug discovery and the life sciences. Among these are the need to extract and amplify RNA, the difficulty and unreliability of measuring gene expression from fixed tissue or whole blood, the lack of reproducibility between sample measurements, and day-to-day repeatability. Performance has not been even close to that of biochemical enzyme and receptor assays, which have therefore remained the mainstay of modern drug discovery. These limitations hinder the exploitation of polymerase chain reaction (PCR) for drug discovery and the power of whole-genome high-density ...

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