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Article: [.sup.31]P NMR study of GTP-cation interactions.(nuclear magnetic resonance)
- Article from:
- Georgia Journal of Science
- Article date:
- December 22, 2005
- Author:
CopyrightCOPYRIGHT 2005 Georgia Academy of Science. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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ABSTRACT
Guanosine 5'-triphosphate (GTP) plays a pivotal role in many
biological processes. The interactions of cations with GTP are
essential to the stabilization of this nucleotide and the
regulation of its functions. In this study, we present solution
[.sup.31]P NMR data on GTP samples in varied salt concentrations
or pHs. From chemical shifts and [T.sub.1], [T.sub.2] relaxations,
it is concluded that the interaction strengths of cations with GTP
is in the order of C[a.sup.2+] > M[g.sup.2+] > L[i.sup.+] >
N[a.sup.+] for the cations, and in the order of [beta] > [gamma] >
[alpha] for the phosphate groups; An increased ...