Article: Increased image depth for epi-illumination microscopy.(NOTE)

Abstract: Epi-illumination microscopy is a useful tool for studying morphology, but its use is limited by a small depth of field. Using digital editing, we have enhanced the depth of field for epi-illumination micrographs obtained for a number of shoot apices. For each specimen, an epi-illumination microscope fitted with Leitz Ultropak dipping cones was used to capture a series of digital images at successive levels of focus. Adobe1 Photoshop1 was used to produce one image by assembling only the focused areas of the image series. This stacking method offers a better way to present epi-illumination micrographs and increases the microscope's usefulness as an alternative for ...

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