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Article: A method to select for mutator DNA polymerase [delta]s in Saccharomyces cerevisiae.(TECHNIQUE)
- Article from:
- Genome
- Article date:
- April 1, 2006
- Author:
CopyrightCOPYRIGHT 2006 NRC Research Press. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Abstract: Proofreading DNA polymerases share common short peptide motifs that bind [Mg.sup.2+] in the exonuclease active center; however, hydrolysis rates are not the same for all of the enzymes, which indicates that there are functional and likely structural differences outside of the conserved residues. Since structural information is available for only a few proofreading DNA polymerases, we developed a genetic selection method to identify mutant alleles of the POL3 gene in Saccharomyces cerevisiae, which encode DNA polymerase [delta] mutants that replicate DNA with reduced fidelity. The selection procedure is based on genetic methods used to identify "mutator" DNA ...