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Article: Subspecies characterization of urease-positive thermophilic Campylobacter (UPTC) isolated from shellfish employing modified flagellin (flaA) restriction fragment length polymorphism (RFLP) typing.
- Article from:
- Journal of Shellfish Research
- Article date:
- August 1, 2006
- Author:
CopyrightCOPYRIGHT 2006 National Shellfisheries Association, Inc. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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ABSTRACT Shellfish including oysters (Crassostrea gigas), cockles (Cerastoderma edule) and mussels (Mytilus edulis), have previously been described as an important source of thermophilic campylobacters, with the potential of causing acute bacterial gastroenteritis in humans. Previous genotyping studies employing the polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP) typing, based on the flagellin (flaA) gene have been unable to generate an amplicon for the urease-positive thermophilic Campylobacter (UPTC), which are the predominant taxa associated with shellfish, largely caused by sequence diversity between the UPTC group and C. jejuni. Hence ...