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Article: Inducción de callo embriogenico en eucalyptus globulus labill.
- Article from:
- Interciencia
- Article date:
- October 1, 2006
- Author:
CopyrightCOPYRIGHT 2006 Interciencia Association. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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SUMMARY
Embryogenic callus induction starting from three explants coming from mature seeds (mature zygotic embryos, hypocotils and cotyledons) was studied. The results indicated that the medium 13 (B5 + ANA 0.5mg x [l.sup.-1] + BAP 1.0mg x [l.sup.01]) produced the largest percentages of callus formation in all the explants. Micromorphology revealed the presence of embryogenic cells in all explants, while globular states in initial phases were found in hypocotils and cotyledons. Later, in the differentiation phase, a maximum of 25 globular states by eallus were obtained in a B5 medium with maltose (2% p/v) added, when using mature zygotic embryos. The histological ...
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