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Article: Profiles of global gene expression in ionizing-radiation--damaged human diploid fibroblasts reveal synchronization behind the [G.sub.1] checkpoint in a [G.sub.0]-like state of quiescence.(Research)
- Article from:
- Environmental Health Perspectives
- Article date:
- April 1, 2006
- Author:
CopyrightCOPYRIGHT 2006 National Institute of Environmental Health Sciences. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Cell cycle arrest and stereotypic transcriptional responses to DNA damage induced by ionizing radiation (IR) were quantified in telomerase-expressing human diploid fibroblasts. Analysis of cytotoxicity demonstrated that 1.5 Gy IR inactivated colony formation by 40-45% in three fibroblast lines; this dose was used in all subsequent analyses. Fibroblasts exhibited > 90% arrest of progression from [G.sub.2] to M at 2 hr post-IR and a similarly severe arrest of progression from [G.sub.1] to S at 6 and 12 hr post-IR. Normal rates of DNA synthesis and mitosis 6 and 12 hr post-IR caused the S and M compartments to empty by > 70% at 24 hr. Global gene expression was analyzed in ...