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Article: The DNA polymerase activity of Pol [epsilon] holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts.
- Article from:
- BMC Biochemistry
- Article date:
- December 1, 2006
- Author:
CopyrightCOPYRIGHT 2006 BioMed Central Ltd. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Abstract
Background: DNA polymerase [epsilon] (Pol [epsilon]) is involved in DNA replication, repair, and cell-cycle checkpoint control in eukaryotic cells. Although the roles of replicative Pol [alpha] and Pol [delta] in chromosomal DNA replication are relatively well understood and well documented, the precise role of Pol [epsilon] in chromosomal DNA replication is not well understood.
Results: This study uses a Xenopus egg extract DNA replication system to further elucidate the replicative role(s) played by Pol [epsilon]. Previous studies show that the initiation timing and elongation of chromosomal DNA replication are markedly impaired in Pol ...