Article: High-resolution measurement of the time course of calcium-concentration microdomains at squid presynaptic terminals.

The spontaneous quantum emission domains (QEDs) observed prior to stimulation were imaged over an area corresponding to [is approximately equal to]20% of the total active zone of the preterminal studied. They occurred at a very low rate (1015/s) and were generally located at the same sites as the evoked microdomains.

Calcium entry following tetanic stimulation of the presynaptic axon (500 ms or 1 s at 100 Hz) was characterized by the appearance of calcium microdomain sites that appeared to blink on and off during the tetanic stimulation. The localization of the evoked microdomains in the presynaptic terminal is shown in Fig. IA, and as previously reported (5), ...

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