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Oligonucleotide delivery with serum into HeLa cells using polycation liposomes.(Clinical report)
- Article from:
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Journal of Applied Research
- Article date:
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March 1, 2007
- Author:
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Copyright informationCOPYRIGHT 2007 Therapeutic Solutions LLC. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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ABSTRACT
Antisense oligonucleotides (ON) are promising novel therapeutic agents against viral infections and cancer. Polycations have gained increasing attention as nonviral gene delivery vectors in the past decades. Significant progress has been made in understanding complex formation between polycations and nucleic acids, entry of the complex into the cells, and subsequent entry into the nucleus. There is an efficient, systemic transgene expression in many cell lines (in vitro) by using anionic liposomes (AL), composed of equimolar amounts of 1. 2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE), dioleoyl phosphatidyl serine (DOPS) and cholesterol (CHOL). These ...
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