Article: Development of a microbiological assay to determine the potency of ceftiofur sodium powder.(VETERINARY DRUG RESIDUES)(Report)

Ceftiofur sodium is a third-generation broad-spectrum cephalosporin antibiotic, formulated as an intramuscular injection, that is approved for use in pigs, cattle, poultry, and dogs. The present work reports a method to quantify ceftiofur in powder for injection by comparing the cylinder plate assay and the liquid chromatographic (LC) method. The assay is based on the inhibitory effect of ceftiofur upon the strain of Micrococcus luteus ATCC 10240 used as the test microorganism. Ceftiofur sodium at concentrations ranging from 2.0 to 8.0 [micro]g/mL can be measured in powder for injection. A prospective validation showed that the method is linear ([r.sup.2] = 0.9998), with ...

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