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Article: Stigma receptivity and in vitro citrus pollen grains germination protocol adjustment/Receptividad del estigma y ajuste del protocolo para la germinacion in vitro de granos del polen en citricos/Receptividade do estigma e ajuste de protocolo para germinacao in vitro de graos de polen de citros.(REPORTS/COMUNICIONES/COMUNICACOES)
- Article from:
- Interciencia
- Article date:
- January 1, 2008
- Author:
CopyrightCOPYRIGHT 2008 Interciencia Association. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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SUMMARY
Data about the viability and germination of pollen grains are essential for the success of on-field controlled hybridizing. The purpose of this work was to study the stigmas receptivity period of citrus flowers under different stages and adjust the protocol to the in vitro pollen grain germination. Six essays were carried out with the aim to define the stages for collection and pollinizations, and to establish the pH and ideal sucrose, agar, calcium and boron concentrations, and temperature, for germination.
For the Pera, Natal and Valencia sweet orange varieties, manual pollinization can be performed using flowers at the "balloon" stage. For ...
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