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Article: Time-resolved analysis of a highly sensitive Forster Resonance Energy Transfer immunoassay using terbium complexes as donors and quantum dots as acceptors.(Research Article)
- Article from:
- Journal of Biomedicine and Biotechnology
- Article date:
- January 1, 2007
- Author:
CopyrightCOPYRIGHT 2007 Hindawi Publishing Corp. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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CdSe/ZnS core/shell quantum dots (QDs) are used as efficient Forster Resonance Energy Transfer (FRET) acceptors in a time-resolved immunoassays with Tb complexes as donors providing a long-lived luminescence decay. A detailed decay time analysis of the FRET process is presented. QD FRET sensitization is evidenced by a more than 1000-fold increase of the QD luminescence decay time reaching ca. 0.5 milliseconds, the same value to which the Tb donor decay time is quenched due to FRET to the QD acceptors. The FRET system has an extremely large Forster radius of approx. 100 [degrees]A and more than 70% FRET efficiency with a mean donor-acceptor distance of ca. 84 [degrees] A, ...
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