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Article: Evaluation of fecal dna purification methods for the detection of E. coli O157:H7 in feces of naturally infected feedlot cattle.(COMMUNICATIONS--GRADUATE)(Report)
- Article from:
- Proceedings of the North Dakota Academy of Science
- Article date:
- April 1, 2008
- Author:
CopyrightCOPYRIGHT 2008 North Dakota Academy of Science. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Several protocols have been used to purify DNA from animal feces for PCR; direct DNA extraction from feces, DNA extraction with previous enrichment in selective media, and the use of commercial kits that involve the use of spin columns. The objective of this study was to compare the accuracy of the QIAamp DNA Stool Mini Ktt (QIAamp Kit) in detecting E. coli O157:H7 in enrichment and non-enrichment cattle feces from naturally infected feedlot cattle.
[FIGURE 1 OMITTED]
Using conventional culture methods (gold standard), 456 fecal samples were analyzed for detectable levels of E. coli O157:H7. QIAamp Kit was used to purify fecal DNA (from enrichment and ...