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Article: Spreeta-based biosensor immunoassays to detect fraudulent adulteration in milk and milk powder.(SPECIAL GUEST EDITOR SECTION)(Report)
- Article from:
- Journal of AOAC International
- Article date:
- May 1, 2006
- Author:
CopyrightCOPYRIGHT 2006 AOAC International. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Biacore biosensors (Biacore AB, Uppsala, Sweden) have proven to be robust analytical tools for the automated immunochemical detection of different adulterants and contaminants in milk and milk powder. However, the significant cost of the instruments is a disincentive for their wide application in food control laboratories. Therefore, a low-cost alternative optical biosensor (Spreeta[TM], Texas Instruments, Attleboro, MA) was built into an affordable liquid handling system. Using this prototype biosensor, an inhibition immunoassay for bovine [kappa]-casein was evaluated for the detection of cow's milk in ewe's and goat's milk and for the detection of bovine rennet whey ...
<0.1%) and fast run times (around 5 min) were obtained with minimal sample preparation. Advantages of the direct assays were described as a single reagent format (biosensor chip-immobilized antibodies only), the use of small amounts of antibodies (2 [micro]g for>