|
|
Article: Characterization of cDNA of lycopene [beta]-cyclase responsible for a high level of [beta]-carotene accumulation in Dunaliella salina.(COMMUNICATION)(complementary deoxyribonucleic acid)
- Article from:
- Biochemistry and Cell Biology
- Article date:
- June 1, 2008
- Author:
CopyrightCOPYRIGHT 2008 NRC Research Press. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
|
Abstract: Lycopene [beta]-cyclase (Lyc-B) is the key enzyme in the catalysis of linear lycopene to form cyclic [beta]- carotene, an indispensable part of the photosynthetic apparatus and an important source of vitamin A in human and animal nutrition. Studies showing that the microalga Dunaliella salina can accumulate a high level of [beta]-carotene are lacking. We hypothesize that D. salina is closely involved with the catalytic mechanism of Lyc-B and the molecular regulation of its gene. In this study, we used RT-PCR and RACE-PCR to isolate a 2475 by cDNA with a 1824 by open reading frame, encoding a putative Lyc-B, from D. salina. Homology studies showed that the deduced ...