|
|
Article: Determination of vitamin [B.sub.12] in food products by liquid chromatography/UV detection with immunoaffinity extraction: single-laboratory validation.(FOOD COMPOSITION AND ADDITIVES)(ultraviolet)(Report)
- Article from:
- Journal of AOAC International
- Article date:
- July 1, 2008
- Author:
CopyrightCOPYRIGHT 2008 AOAC International. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
|
A fast and simple method to determine vitamin [B.sub.12] in foods is presented. The method allows, in addition to the determination of added cyanocobalamin, the determination of natural vitamin [B.sub.12] forms, making it also applicable to nonfortified products, especially those that are milk-based. Vitamin [B.sub.12] is extracted in sodium acetate buffer in the presence of sodium cyanide (100[degrees]C, 30 min). After purification and concentration with an immunoaffinity column, vitamin [B.sub.12] is determined by liquid chromatography with UV detection (361 nm). The method has been validated in analyses of a large range of products: milk- and soy-based infant formulas, ...