Article: Determination of vitamin [B.sub.12] in food products by liquid chromatography/UV detection with immunoaffinity extraction: single-laboratory validation.(FOOD COMPOSITION AND ADDITIVES)(ultraviolet)(Report)

A fast and simple method to determine vitamin [B.sub.12] in foods is presented. The method allows, in addition to the determination of added cyanocobalamin, the determination of natural vitamin [B.sub.12] forms, making it also applicable to nonfortified products, especially those that are milk-based. Vitamin [B.sub.12] is extracted in sodium acetate buffer in the presence of sodium cyanide (100[degrees]C, 30 min). After purification and concentration with an immunoaffinity column, vitamin [B.sub.12] is determined by liquid chromatography with UV detection (361 nm). The method has been validated in analyses of a large range of products: milk- and soy-based infant formulas, ...

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