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Article: Research from L.S. Lima and co-researchers provides new data on molecular breeding.
- Article from:
- Life Science Weekly
- Article date:
- September 2, 2008
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According to a study from Brazil, "Theobroma cacao L.-Moniliophthora perniciosa expressed sequence tags (ESTs) were converted into useful satellite markers for population analysis and genetic mapping. Forty-nine flanking primer pairs from TSH1188 (a resistant genotype) and Catongo (a susceptible genotype) ESTs were designed and screened for polymorphism analysis."
"Eleven were polymorphic, with an average of 3.81 alleles per locus and a total of 42 alleles. The satellite markers were tested on 21 cacao accessions and two bulked DNAs generated from 6 resistant and 6 susceptible plants from a segregating F-2 (SCA6 x ICS1) population for witches' broom resistance," ...