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Article: Research from V.L. Boyd and co-authors in the area of capillary electrophoresis published.
- Article from:
- Biotech Week
- Article date:
- October 15, 2008
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"Polymerase-mediated single-base extension (SBE) of primers using a fluorescently labeled 2',3'-dideoxynucleotide triphosphate terminator was originally commercialized as SNaPshot for analysis of single-nucleotide polymorphisms by capillary electrophoresis (CE)," investigators in the United States report (see also Capillary Electrophoresis).
"Application of this general method to bisulfite-converted/PCR-amplified genomic DNA (gDNA) to analytically infer polymorphic methylation status (i.e., 5-methylcytosine [5mC] vs. cytosine [C]) in CpG-rich regions of gDNA has been noted previously by others to be limited by CE mobility-shifted peaks for SBE products derived ...