Article: AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images.(Fluorescence resonance energy transfer)(Software)(Report)

Authors: J ános Roszik [1]; János Szöll?si [1]; György Vereb (corresponding author) [1]

Background

Fluorescence resonance energy transfer (FRET) is a powerful technique that can be applied to study nanoscale intra- and intermolecular events and interactions of molecules in situ in biological systems [1]. In assessing FRET, fluorescence of a spectrally matched donor and acceptor dye pair can be measured to reveal the radiationless transfer of excitation energy from the donor to the acceptor, in the case that their dipoles are properly oriented and the two are in spatial proximity (usually at a ...

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