Article: Research from University of Stuttgart yields new data on enzyme research.

According to recent research published in the journal Proteins - Structure Function and Bioinformatics, "The molecular basis of regioselectivity of cytochrome P450 monooxygenases from Bacillus megaterium (CYPI02A1) with its flexible and widely opened active site is still not well understood. In the present work (-)-alpha-pinene bound complexes with two triple mutants were modeled to elucidate the contribution of the three major factors that mediate selectivity: active site shape, protein flexibility, and chemical reactivity of the substrate."

"For the triple mutant A74G F87V L188Q (GVQ), one stable, productive conformation of the substrate (conformation I) was ...

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