|
|
Article: Linkage mapping of developmentally expressed cDNAS containing AT-rich elements in shrimp (Litopenaeus vannamei)--differential expression after challenge with Taura Syndrome virus (TSV).(Report)
- Article from:
- Journal of Shellfish Research
- Article date:
- December 1, 2007
- Author:
CopyrightCOPYRIGHT 2007 National Shellfisheries Association, Inc. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
|
ABSTRACT To explore the possibility of finding interleukin-like gene(s) in Litopenaeus vannamei shrimp, we sought evidence for the presence of interleukin 1 (IL-1)-like genes, because the proteins encoded by these genes are evolutionarily conserved. RT-PCR performed with primers from conserved regions of vertebrate 1L-1[alpha] genes amplified three bands of 954 bp (ShIL1-21; accession # U94689), 864 bp (ShIL1-28; U94090), and 182 bp (ShIL1-J17; U94691) from various tissues. The cDNA sequences were AT-rich (~65%) and contained 1-5 copies of the "ATTTA" motif, a possible indicator of mRNA degradation. GenBank search indicated that similar AT-rich elements are present in the ...