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Article: A modified suspension test for estimating the mutagenicity of samples containing free and (or) protein-bound histidine.(Report)
- Article from:
- Canadian Journal of Microbiology
- Article date:
- February 1, 2009
- Author:
CopyrightCOPYRIGHT 2009 NRC Research Press. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Introduction
Because of its short period, simplicity, and high sensitivity to mutagens (83%) (Ashby and Tennant 1991; Mortelmans and Zeiger 2000), the Ames test has been widely adopted for the estimation of the mutagenicity of substances in the environment for several decades (Ames et al. 1975; Fluckiger-Isler et al. 2004; Aufderheide and Gressmann 2007).
The Ames test relies on the reversion of auxotrophs to histidine-independent strains owing to the reversion mutation of the genes involved in the biosynthetic pathway (Mortelmans and Zeiger 2000). Since [his.sup.+] revertants are detected by growth in media containing only a low histidine concentration, ...