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Article: Research results from J. Mano and colleagues update understanding of polymerase chain reaction.
- Article from:
- Medical Devices & Surgical Technology Week
- Article date:
- May 17, 2009
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"We developed a multiplex polymerase chain reaction (PCR)-multiplex ligase chain reaction (LCR) (MPCR-MLCR) technique as a novel approach for the simultaneous detection of recombinant DNA segments (e.g., promoters, trait genes, and terminators) of genetically modified (GM) crops. With this technique, target DNA regions were amplified by multiplex PCR, the PCR products were then subjected to multiplex LCR as template DNAs, and the LCR products were then analyzed by polyacrylamide gel electrophoresis and subsequent fluorescent scanning," scientists in Tsukuba, Japan report (see also Polymerase Chain Reaction).
"Seven recombinant DNA segments commonly introduced ...