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Article: ALT1-encoded alanine aminotransferase plays a central role in the metabolism of alanine in Saccharomyces cerevisiae.(Report)
- Article from:
- Canadian Journal of Microbiology
- Article date:
- April 1, 2009
- Author:
CopyrightCOPYRIGHT 2009 NRC Research Press. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Introduction
Pathways for the biosynthesis and catabolism of amino acids have been thoroughly studied in Saccharomyces cerevisiae; however, the exact nature of the routes leading to alanine biosynthesis and catabolism have remained unclear and no alanine auxotrophs have been isolated.
Three major biosynthetic pathways for alanine biosynthesis have been identified in bacteria: reductive amination of pyruvate by alanine dehydrogenase (AlaD) (McCowen and Phibbs 1974; Feng et al. 2002), decarboxylation of i.-aspartate by aspartate-4-decarboxylase (AspD) (Wang and Lee 2006), and transamination from other amino acids to pyruvate via aminotransferases (Wang et ...