|
|
Article: Design and testing of real-time PCR primers for the quantification of Methanoculleus, Methanosarcina, Methanothermobacter, and a group of uncultured methanogens.(NOTE)(polymerase chain reaction)(Report)
- Article from:
- Canadian Journal of Microbiology
- Article date:
- May 1, 2009
- Author:
CopyrightCOPYRIGHT 2009 NRC Research Press. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
|
In recent years, the advent and application of molecular technologies have resulted in significant advances in the field of microbial ecology. Such technologies have allowed uncultured and, thus, previously unknown microorganisms to be studied. The direct extraction of DNA from an environmental sample and the subsequent amplification of the 16S rRNA genes by PCR have contributed significantly to ecological studies, with different methods of microbial community fingerprinting sharing this strategy as a starting point. The 16S rRNA gene is present in all prokaryotic cells, and it contains both variable and conserved regions that allow the development of phylogenetically ...