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Article: Research reports from Hospital for Sick Children provide new insights into proteomics.
- Article from:
- Proteomics Weekly
- Article date:
- June 15, 2009
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"Cystic Fibrosis is caused by mutations in CFTR, with a deletion of a phenylalanine at position 508 (F508del-CFTR) representing the most common mutation. The F508del-CFTR protein exhibits a trafficking defect and is retained in the endoplasmic reticulum," scientists in Toronto, Canada report (see also Proteomics).
"Here we describe the development of a high-content screen based on a functional assay to identify proteins that correct the F508del-CFTR defect. Using a HEK293 MSR GripTite cell line that stably expresses F508del-CFTR, we individually co-expressed similar to 450 unique proteins fused to the Cl--sensitive YFP(H148Q/I152L) mutant. We then tested ...