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Article: Investigators at University of Ghent zero in on applied microbiology.
- Article from:
- Science Letter
- Article date:
- June 16, 2009
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According to a study from Ghent, Belgium, "Comparison of an internally-controlled real-time PCR assay with the current plate-based assay for the detection of Bacillus sensu lato contaminants in gelatine. A comprehensive TaqMan((R)) probe was designed allowing the real-time PCR assay to be fully inclusive for the gelatine-contaminating Bacillus s.l. species."
"An internal amplification control was implemented at 500 copies per reaction without impact on target detection. Specific and selective detection of target cells was achieved with a quick and simple DNA preparation procedure. No significant difference (Kappa value = 0.94) was observed between the performance ...
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