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Article: University of Colorado details research in chromatography.
- Article from:
- Proteomics Weekly
- Article date:
- March 30, 2009
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"A simple, selective, and sensitive LC-MS/MS method was developed for the simultaneous extraction and determination of eight beta-blockers (atenolol, sotalol, nadolol, pindolol, timolol, metoprolol, betaxolol and propranolol) in various bovine eye tissues including sclera, choroid-RPE, retina, and vitreous. The analytes were extracted by liquid-liquid extraction after samples were alkalinized with 2% NaOH solution in water," investigators in the United States report (see also Enzyme Research).
"The chromatographic separation was performed on a Hypersil-ODS C18 column (100 mm x 2.1 mm, 3.9 mu m) using a gradient mixture of (A) 5 mM ammonium formate in water (pH ...