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Article: Studies from University of Rostock, Proteome Center Rostock update current data on arthritis.
- Article from:
- Biotech Week
- Article date:
- October 7, 2009
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"Identification of proteins from apheresis samples was performed by both SDS-PAGE and 2-D gel separation of eluted proteins from staphylococcal protein A-based immunoadsorption columns (Prosorba [R]) followed by MS peptide mass fingerprinting and MS/MS peptide sequencing on a MALDI QIT TOF mass spectrometer. MS/MS peptide sequencing was performed in conjunction with a micro reversed phase HPLC configured with an online MALDI plate-spotting device," investigators in Rostock, Germany report (see also Arthritis).
"Apheresis treatment had been performed in three patients with longstanding therapy refractory rheumatoid arthritis. 2-D gels displayed ca. 500 spots ...