Article: Researchers at University of Colorado target life sciences.

"The FYVE domain associates with phosphatidylinositol 3-phosphate [PtdIns(3)P] in membranes of early endosomes and penetrates bilayers. Here, we detail principles of membrane anchoring and show that the FYVE domain insertion into PtdIns(3)P-enriched membranes and membrane-mimetics is substantially increased in acidic conditions," scientists writing in the journal Proteins - Structure Function and Bioinformatics report (see also Life Sciences).

"The EEA1 FYVE domain binds to POPC/POPE/PtdIns(3)P vesicles with a Kd of 49 nM at pH 6.0, however associates similar to 24 fold weaker at pH 8.0. The decrease in the affinity is primarily due to much faster dissociation of ...

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