Article: New findings reported from Royal Manchester Children's Hospital describe advances in cancer therapy.(Report)

According to recent research from Manchester, the United Kingdom, "The aim of this study was to investigate the effects of methionine depletion with cytotoxic agents that are potentially influenced by depletion of methionine, and are known to have a role in CNS tumour treatments for children. Cytotoxicity studies and synergistic interactions were assayed by SRB assay."

"Glutathione levels were assayed by HPLC after derivatization with OPA. MGMT activity was determined by a restriction endonuclease inhibition assay. Methionine depletion causes a demonstrable increase in glutathione levels for medulloblastoma (Daoy) and glioma (D54) cells, with a decrease in MGMT ...

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