Article: New biological chemistry findings from Iwate Medical University published.

"The temperature-dependent rotation of F-1-ATPase gamma subunit was observed in V-max conditions at low viscous drag using a 60-nm gold bead (Nakanishi-Matsui, M., Kashiwagi, S., Hosokawa, H., Cipriano, D. J., Dunn, S. D., Wada, Y., and Futai, M. (2006) J. Biol. Chem. 281, 4126-4131). The Arrhenius slopes of the speed of the individual 120 degrees steps and reciprocal of the pause length between rotation steps were very similar, indicating a flat energy pathway followed by the rotationally coupled catalytic cycle," investigators in Japan report.

"In contrast, the Arrhenius slope of the reciprocal pause length of the gamma M23K mutant F-1 was significantly ...

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