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Article: Research from University of Ottawa yields new findings on helicobacter pylori.
- Article from:
- Biotech Week
- Article date:
- November 4, 2009
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According to a study from Ottawa, Canada, "Mutations at positions 2142 or 2143 in the two-copy 23S ribosomal RNA gene of Helicobacter pylori are highly predictive of in vitro clarithromycin resistance and failure of clarithromycin-containing treatment regimens. To design an assay to rapidly detect these Mutations using rapid polymerase chain reaction and pyrosequencing, a novel method of 'sequencing by synthesis', and to test this assay with a collection of Canadian H pylori isolates."
"Forty-two H pylori isolates (24 clarithromycin-resistant, 18 clarithromycin-susceptible) were studied. A target region in the 23S gene was rapidly amplified and sequenced by ...