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Article: New findings from Food Research Institute in the area of polymerase chain reaction published.
- Article from:
- Medical Devices & Surgical Technology Week
- Article date:
- November 8, 2009
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Data detailed in 'A single-tube nested real-time polymerase chain reaction for sensitive contained detection of Cryptosporidium parvum' have been presented. "A new real-time polymerase chain reaction (PCR) was developed for sensitive contained detection of Cryptosporidium parvum. The method is a nested PCR targeting a specific region of rDNA of C. parvum, which takes place in one tube, using different annealing temperatures to control the first and the second rounds of PCR, with real-time fluorogenic probe-based detection of the second round of PCR," investigators in Bratislava, Slovakia report (see also Polymerase Chain Reaction).
"The DNA-based detection limit ...