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Article: Studies in the area of forestry reported from University of Hamburg.
- Article from:
- Agriculture Week
- Article date:
- November 5, 2009
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According to recent research from Hamburg, Germany, "A molecular technique was used to detect the bacterium Pseudomonas syringae pv. aesculi in horse chestnut trees (Aesculus hippocastanum), affected by the recently recognized European 'Pseudomonas horse chestnut bark disease'. The technique helped identify the pathogen within 6 h of sample preparation including DNA extraction, polymerase chain reaction (PCR) and electrophoresis until gel documentation."
"PCR primer pairs derived from the gyrase B gene sequence were used. Because of the great similarity in the gyrase B gene sequences of the numerous closely related P. syringae pathovars, the primers were not only ...
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