|
|
Article: Research results from National Institutes of Health update understanding of DNA research.
- Article from:
- Biotech Week
- Article date:
- November 11, 2009
CopyrightCOPYRIGHT 2009 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
|
According to a study from the United States, "Two different microenvironments in the DNA sequence 5'-act aGa gat ccc tca gac cct ttt agt cag tGt gga-3' (in both single-and double-stranded forms) are explored using two similar fluorescent nucleoside analogues, 3MI and 6MI. Each probe was evaluated in two environments, one strand with the probe flanked by thymines (PTRT) and the other by adenines (PTRA) with positions indicated by G's in the sequence."
"Both time-resolved anisotropies and lifetimes of the probes depend upon local interactions, and these are altered by duplex formation. Integrals of lifetime curves compared with quantum yields reveal that each probe ...
<70 ps). For 6MI in PTRA, this QSSQ ''quasi-static self quenching'' or ''dark'' component represents approximately half the Molecules, whether in single- or double-stranded form. In PTRT, 6MI displays an unusual increase in the quantum yield upon formation of the double strand (from 0.107 to 0.189) apparently the result of escape from QSSQ which simultaneously declines from 66 to 33%. This is also accompanied by doubling of steady-state anisotropy. Only 6MI in the PTRT duplex displays a rotational correlation time of>
Related newspaper, magazine, and journal articles:
|
|
Article: Scientists at National Institutes of Health discuss ...
Biotech Week;
July 1, 2009 ;
623 words
... ... colleagues, National Institutes of Health (see also DNA Research). The researchers ... Hinnebusch, National Institutes of Health ... Replication, DNA Research, Deoxyribonucleic ... Ribosome, National Institutes of Health ...
|
|