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Article: Reports on protease findings from S.Y. Guo and co-researchers provide new insights.
- Article from:
- Biotech Week
- Article date:
- November 11, 2009
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"Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion," investigators in Beijing, People's Republic of China report (see also Protease).
"The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers," wrote S.Y. Guo and colleagues.
The researchers concluded: "The 50% lethal concentrations (LC50) of the ...