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Article: New Escherichia coli research has been reported by scientists at University of Missouri.(Report)
- Article from:
- Medical Letter on the CDC & FDA
- Article date:
- November 15, 2009
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According to a study from the United States, "The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR."
"Samples were stained with EMA for 5 min, iced for 1 min and exposed to bright visible light for 10 min prior to DNA extraction, to allow EMA binding of the DNA from dead cells. DNA was then extracted and amplified by TaqMan [R] real-time PCR to detect only viable E. coli O157:H7 cells. The primers and TaqMan [R] ...
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